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Violin box plot showing the percentage of differentiated astrocytes (NDRG2+) that express the reactive astrocytes biomarkers pSTAT3 ( A ) and <t>NFκβ</t> <t>p65</t> ( E ) in cortical tissue from controls (n = 12) and DR-TLE patients (pSTAT3: n = 17; NFκβ p65: n = 16). B-D) Percentage of astrocytes co-expressing NDRG2 and pSTAT3 classified according to their DNA content. B ) % 2C astrocytes; C ) % 4C astrocytes; D ) % >4C astrocytes. F-H) Percentage of astrocytes co-expressing NDRG2 and NFκβ p65 classified according to their DNA content. F ) % 2C astrocytes; G ) % 4C astrocytes; H ) % >4C astrocytes. **p < 0.01, ***p < 0.001. Abbreviations: DR-TLE: drug-resistant temporal lobe epilepsy; NDRG2: N-Myc Downstream-Regulated Gene 2 Protein; p65: <t>p65</t> <t>subunit</t> of nuclear factor-κβ; pSTAT3: phosphorylated Signal Transducer and Activator of Transcription 3; 2C: diploid; 4C: tetraploid; >4C: Polyploid with a DNA content higher than 4C.
Mouse Monoclonal Anti Nfκβ P65, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp rela rn01502266 m1  (Thermo Fisher)
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Violin box plot showing the percentage of differentiated astrocytes (NDRG2+) that express the reactive astrocytes biomarkers pSTAT3 ( A ) and <t>NFκβ</t> <t>p65</t> ( E ) in cortical tissue from controls (n = 12) and DR-TLE patients (pSTAT3: n = 17; NFκβ p65: n = 16). B-D) Percentage of astrocytes co-expressing NDRG2 and pSTAT3 classified according to their DNA content. B ) % 2C astrocytes; C ) % 4C astrocytes; D ) % >4C astrocytes. F-H) Percentage of astrocytes co-expressing NDRG2 and NFκβ p65 classified according to their DNA content. F ) % 2C astrocytes; G ) % 4C astrocytes; H ) % >4C astrocytes. **p < 0.01, ***p < 0.001. Abbreviations: DR-TLE: drug-resistant temporal lobe epilepsy; NDRG2: N-Myc Downstream-Regulated Gene 2 Protein; p65: <t>p65</t> <t>subunit</t> of nuclear factor-κβ; pSTAT3: phosphorylated Signal Transducer and Activator of Transcription 3; 2C: diploid; 4C: tetraploid; >4C: Polyploid with a DNA content higher than 4C.
Gene Exp Rela Rn01502266 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Violin box plot showing the percentage of differentiated astrocytes (NDRG2+) that express the reactive astrocytes biomarkers pSTAT3 ( A ) and <t>NFκβ</t> <t>p65</t> ( E ) in cortical tissue from controls (n = 12) and DR-TLE patients (pSTAT3: n = 17; NFκβ p65: n = 16). B-D) Percentage of astrocytes co-expressing NDRG2 and pSTAT3 classified according to their DNA content. B ) % 2C astrocytes; C ) % 4C astrocytes; D ) % >4C astrocytes. F-H) Percentage of astrocytes co-expressing NDRG2 and NFκβ p65 classified according to their DNA content. F ) % 2C astrocytes; G ) % 4C astrocytes; H ) % >4C astrocytes. **p < 0.01, ***p < 0.001. Abbreviations: DR-TLE: drug-resistant temporal lobe epilepsy; NDRG2: N-Myc Downstream-Regulated Gene 2 Protein; p65: <t>p65</t> <t>subunit</t> of nuclear factor-κβ; pSTAT3: phosphorylated Signal Transducer and Activator of Transcription 3; 2C: diploid; 4C: tetraploid; >4C: Polyploid with a DNA content higher than 4C.
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Violin box plot showing the percentage of differentiated astrocytes (NDRG2+) that express the reactive astrocytes biomarkers pSTAT3 ( A ) and <t>NFκβ</t> <t>p65</t> ( E ) in cortical tissue from controls (n = 12) and DR-TLE patients (pSTAT3: n = 17; NFκβ p65: n = 16). B-D) Percentage of astrocytes co-expressing NDRG2 and pSTAT3 classified according to their DNA content. B ) % 2C astrocytes; C ) % 4C astrocytes; D ) % >4C astrocytes. F-H) Percentage of astrocytes co-expressing NDRG2 and NFκβ p65 classified according to their DNA content. F ) % 2C astrocytes; G ) % 4C astrocytes; H ) % >4C astrocytes. **p < 0.01, ***p < 0.001. Abbreviations: DR-TLE: drug-resistant temporal lobe epilepsy; NDRG2: N-Myc Downstream-Regulated Gene 2 Protein; p65: <t>p65</t> <t>subunit</t> of nuclear factor-κβ; pSTAT3: phosphorylated Signal Transducer and Activator of Transcription 3; 2C: diploid; 4C: tetraploid; >4C: Polyploid with a DNA content higher than 4C.
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Violin box plot showing the percentage of differentiated astrocytes (NDRG2+) that express the reactive astrocytes biomarkers pSTAT3 ( A ) and <t>NFκβ</t> <t>p65</t> ( E ) in cortical tissue from controls (n = 12) and DR-TLE patients (pSTAT3: n = 17; NFκβ p65: n = 16). B-D) Percentage of astrocytes co-expressing NDRG2 and pSTAT3 classified according to their DNA content. B ) % 2C astrocytes; C ) % 4C astrocytes; D ) % >4C astrocytes. F-H) Percentage of astrocytes co-expressing NDRG2 and NFκβ p65 classified according to their DNA content. F ) % 2C astrocytes; G ) % 4C astrocytes; H ) % >4C astrocytes. **p < 0.01, ***p < 0.001. Abbreviations: DR-TLE: drug-resistant temporal lobe epilepsy; NDRG2: N-Myc Downstream-Regulated Gene 2 Protein; p65: <t>p65</t> <t>subunit</t> of nuclear factor-κβ; pSTAT3: phosphorylated Signal Transducer and Activator of Transcription 3; 2C: diploid; 4C: tetraploid; >4C: Polyploid with a DNA content higher than 4C.
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Violin box plot showing the percentage of differentiated astrocytes (NDRG2+) that express the reactive astrocytes biomarkers pSTAT3 ( A ) and <t>NFκβ</t> <t>p65</t> ( E ) in cortical tissue from controls (n = 12) and DR-TLE patients (pSTAT3: n = 17; NFκβ p65: n = 16). B-D) Percentage of astrocytes co-expressing NDRG2 and pSTAT3 classified according to their DNA content. B ) % 2C astrocytes; C ) % 4C astrocytes; D ) % >4C astrocytes. F-H) Percentage of astrocytes co-expressing NDRG2 and NFκβ p65 classified according to their DNA content. F ) % 2C astrocytes; G ) % 4C astrocytes; H ) % >4C astrocytes. **p < 0.01, ***p < 0.001. Abbreviations: DR-TLE: drug-resistant temporal lobe epilepsy; NDRG2: N-Myc Downstream-Regulated Gene 2 Protein; p65: <t>p65</t> <t>subunit</t> of nuclear factor-κβ; pSTAT3: phosphorylated Signal Transducer and Activator of Transcription 3; 2C: diploid; 4C: tetraploid; >4C: Polyploid with a DNA content higher than 4C.
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Violin box plot showing the percentage of differentiated astrocytes (NDRG2+) that express the reactive astrocytes biomarkers pSTAT3 ( A ) and <t>NFκβ</t> <t>p65</t> ( E ) in cortical tissue from controls (n = 12) and DR-TLE patients (pSTAT3: n = 17; NFκβ p65: n = 16). B-D) Percentage of astrocytes co-expressing NDRG2 and pSTAT3 classified according to their DNA content. B ) % 2C astrocytes; C ) % 4C astrocytes; D ) % >4C astrocytes. F-H) Percentage of astrocytes co-expressing NDRG2 and NFκβ p65 classified according to their DNA content. F ) % 2C astrocytes; G ) % 4C astrocytes; H ) % >4C astrocytes. **p < 0.01, ***p < 0.001. Abbreviations: DR-TLE: drug-resistant temporal lobe epilepsy; NDRG2: N-Myc Downstream-Regulated Gene 2 Protein; p65: <t>p65</t> <t>subunit</t> of nuclear factor-κβ; pSTAT3: phosphorylated Signal Transducer and Activator of Transcription 3; 2C: diploid; 4C: tetraploid; >4C: Polyploid with a DNA content higher than 4C.
Recombinant Nf κb Protein, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Violin box plot showing the percentage of differentiated astrocytes (NDRG2+) that express the reactive astrocytes biomarkers pSTAT3 ( A ) and NFκβ p65 ( E ) in cortical tissue from controls (n = 12) and DR-TLE patients (pSTAT3: n = 17; NFκβ p65: n = 16). B-D) Percentage of astrocytes co-expressing NDRG2 and pSTAT3 classified according to their DNA content. B ) % 2C astrocytes; C ) % 4C astrocytes; D ) % >4C astrocytes. F-H) Percentage of astrocytes co-expressing NDRG2 and NFκβ p65 classified according to their DNA content. F ) % 2C astrocytes; G ) % 4C astrocytes; H ) % >4C astrocytes. **p < 0.01, ***p < 0.001. Abbreviations: DR-TLE: drug-resistant temporal lobe epilepsy; NDRG2: N-Myc Downstream-Regulated Gene 2 Protein; p65: p65 subunit of nuclear factor-κβ; pSTAT3: phosphorylated Signal Transducer and Activator of Transcription 3; 2C: diploid; 4C: tetraploid; >4C: Polyploid with a DNA content higher than 4C.

Journal: medRxiv

Article Title: Functional Profiling of Tetraploid Astrocytes in Drug-Resistant Temporal Lobe Epilepsy

doi: 10.64898/2026.01.30.26345206

Figure Lengend Snippet: Violin box plot showing the percentage of differentiated astrocytes (NDRG2+) that express the reactive astrocytes biomarkers pSTAT3 ( A ) and NFκβ p65 ( E ) in cortical tissue from controls (n = 12) and DR-TLE patients (pSTAT3: n = 17; NFκβ p65: n = 16). B-D) Percentage of astrocytes co-expressing NDRG2 and pSTAT3 classified according to their DNA content. B ) % 2C astrocytes; C ) % 4C astrocytes; D ) % >4C astrocytes. F-H) Percentage of astrocytes co-expressing NDRG2 and NFκβ p65 classified according to their DNA content. F ) % 2C astrocytes; G ) % 4C astrocytes; H ) % >4C astrocytes. **p < 0.01, ***p < 0.001. Abbreviations: DR-TLE: drug-resistant temporal lobe epilepsy; NDRG2: N-Myc Downstream-Regulated Gene 2 Protein; p65: p65 subunit of nuclear factor-κβ; pSTAT3: phosphorylated Signal Transducer and Activator of Transcription 3; 2C: diploid; 4C: tetraploid; >4C: Polyploid with a DNA content higher than 4C.

Article Snippet: Mouse monoclonal anti-NFκβ p65 (12054-MM04 Sino Biological, USA) and anti-pSTAT3 (sc-8059 Santa Cruz Biotechnology, USA) antibodies were both diluted 1:500.

Techniques: Expressing

Diagram of the functions played by tetraploid astrocytes. Tetraploid astrocytes express NF1A suggesting that they are involved in electric function. Tetraploid astrocytes present SOX9, a marker of glutamate transporter function and ALDH1L1, an enzyme involved in glucose metabolism. Some of the tetraploid astrocytes have markers of reactive astrocytes (NFκB p65 and pSTAT3). Abbreviation: GLT-1: glutamate transporter 1; NADPH: Nicotinamide adenine dinucleotide phosphate; NDRG2: N-Myc Downstream-Regulated Gene 2 Protein; NF1A: Nuclear factor 1; p65: p65 subunit of nuclear factor-κβ; SOX9: SRY-related high mobility group box gene 9; pSTAT3: phosphorylated Signal Transducer and Activator of Transcription 3; 4C: tetraploid.

Journal: medRxiv

Article Title: Functional Profiling of Tetraploid Astrocytes in Drug-Resistant Temporal Lobe Epilepsy

doi: 10.64898/2026.01.30.26345206

Figure Lengend Snippet: Diagram of the functions played by tetraploid astrocytes. Tetraploid astrocytes express NF1A suggesting that they are involved in electric function. Tetraploid astrocytes present SOX9, a marker of glutamate transporter function and ALDH1L1, an enzyme involved in glucose metabolism. Some of the tetraploid astrocytes have markers of reactive astrocytes (NFκB p65 and pSTAT3). Abbreviation: GLT-1: glutamate transporter 1; NADPH: Nicotinamide adenine dinucleotide phosphate; NDRG2: N-Myc Downstream-Regulated Gene 2 Protein; NF1A: Nuclear factor 1; p65: p65 subunit of nuclear factor-κβ; SOX9: SRY-related high mobility group box gene 9; pSTAT3: phosphorylated Signal Transducer and Activator of Transcription 3; 4C: tetraploid.

Article Snippet: Mouse monoclonal anti-NFκβ p65 (12054-MM04 Sino Biological, USA) and anti-pSTAT3 (sc-8059 Santa Cruz Biotechnology, USA) antibodies were both diluted 1:500.

Techniques: Marker